bacterial proteins中文是什么意思

• 《英文msh词典》Bacterial Proteins
• [主题词] Bacterial Proteins
• [同义词] Bacterial Gene Products
• [同义词] Bacterial Gene Proteins
• [同义词] Gene Products,Bacterial
• [入口词] Bacterial Gene Product
• [入口词] Bacterial Gene Protein
• [入口词] Bacterial Protein
• [入口词] Gene Product,Bacterial
• [入口词] Gene Protein,Bacterial
• [入口词] Gene Proteins,Bacterial
• [入口词] Protein,Bacterial
• [入口词] Proteins,Bacterial
• [中文释义] 细菌蛋白质类
• [英文释义] Proteins found in any species of bacterium.

• bacterial    adj. 细菌的。
• adequate proteins    适宜蛋白, 完全蛋白
• attachment proteins    粘附蛋白
• binding proteins    结合蛋白
• biotinylation of proteins    蛋白质的生物素化
• cationic proteins    阳离子蛋白
• channel proteins    通道蛋白
• complete proteins    完全蛋白质
• conjugated proteins    接合蛋白质; 缀合蛋白质
• cord proteins    脐蛋白
• defensive proteins    布赫内氏体; 防御蛋白
• denaturation of proteins    蛋白质变性作用
• denatured proteins    变性蛋白
• endogenous proteins    内源性蛋白
• fibrous proteins    纤维蛋白
• globular proteins    球蛋白。
• heme proteins    血红素蛋白; 血色素蛋白质
• heteromultimeric proteins    (异源多聚体蛋白质)：有不同的亚基(不同基因编码)组成的蛋白质。
• hmg proteins    hmg 蛋白质
• homologous proteins    同源蛋白质
• homomolecular proteins    等分子量蛋白
• immune proteins    免疫蛋白
• indispensable proteins    必需蛋白
• insoluble proteins    不溶蛋白
• iodized proteins    碘化蛋白

• The amount of the goal protein was evaluated by densitometric scanning . it indicated that the product of the vp2 gene was 14 % of total bacterial protein of dh5a
  表达产物经ni-nta金属鏊合层析柱纯化后，得到了纯度较高的6his-vp2融合蛋白。
• The sds-page analysis revealed that the trka extracellular domains proteins were highly expressed and accumulated up to above 30 % of the total bacterial proteins after iptg induction
  称a膜外域结构域重组蛋白表达量均占全菌蛋白的30%以上。
• The amount of the goal protein was evaluated by densitometric scanning . it indicated that the 45ku product of the vp6 gene was 26.5 % of total bacterial protein of bl21
  表达产物经ni-nta金属螯合层析柱纯化后，得到纯化的his-taggedvp6融合蛋白。
• Especially, the recombinant pet21a having 5 copy gene in e . coli bl21 ( de3 ) got high expression and expression level was up to 25 % of the total bacterial proteins
  其中，含有5份串连基因的pet21a重组质粒在bl21(de3)中的诱导表达量占总蛋白的25左右。
• 3'- utr of h pf4 c dna was deleted and tag was mutated to taataa . 2 after sds-page and densitometric scan analysis, the result show that expression level is 25-30 % of total bacterial proteins
  山西医科大学2002届硕士学位论文一2dhsapbv220rhpf4经温控诱导表达后，sdspage及凝胶密度扫描分析，表达产物占总国体蛋白的2530，凝胶迁移特性与hpf4标准品相同。
• After washing with reagent, adopt the newest purification technology source30rpc, sds-page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins . after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition . finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %
  结论:总之，通过对发酵罐中重组工程菌各种培养因素的研究，建立了一种高密度、高表达发酵工艺体系，为重组蛋白的后续纯化提供了大量、稳定的原料供应;通过对不同目的蛋白的色谱行为的系统研究，建立了一种高效稳定、快速简洁、易于放大的包涵体重组蛋白分离纯化体系。
• In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il-2 gene . about 600 bp chicken il-2 cdna fragment was cloned from cona-stimulated chicken splenocytes by reverse transcription-polymerase chain reaction ( rt-pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next, we construct recombinant plasmid pproex ? t-il-2 . the cdna of chicken il-2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t-il-2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds-page and western blot that a 18kda protein which was equal to chicken il-2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il-2 protein
  经酶切鉴定及dna序列测定，该基因为鸡il-2基因，其序列与sundick等报道的完全一致。在此基础上，我们把鸡il-2基因亚克隆到大肠杆菌原核表达载体pproex~(tm)ht中，构建重组表达质粒并进行确证性序列测定，重组质粒测序结果表明将编码鸡il-2成熟蛋白的基因正确地插入到原核表达载体pproex~(tm)ht的目的位点。重组质粒转化受体菌dh5后用iptg于37进行诱导培养，sds-page和westernblot分析显示，表达的鸡il-2融合蛋白分子量约为18kda，表达的融合蛋白经薄层扫描发现目的蛋白表达量约占菌体蛋白的30。
• Sds-page analysis suggested that the bacteria containing the recombinant plasmid pet-32a ( + )-igf-i produced the fusion protein of 30kda as it was induced by iptg . consisting 10 % of the total bacterial proteins, and the pet-30a ( + )-igf-ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins . 5
  sds-page分析表明，重组质粒pet-32a(+)-igf-在iptg诱导下表达分子量约30kda的融合蛋白，但其表达量不高，约为菌体总蛋白的10左右；重组质粒pet-30a(+)-igf-在iptg诱导下表达分子量约14kda的重组蛋白，融合蛋白表达量约占菌体蛋白总量的35。
• Sds-page analysis suggested that the bacteria containing the recombinant plasmid pet-32a ( + )-igf-i produced the fusion protein of 30kda as it was induced by iptg . consisting 10 % of the total bacterial proteins, and the pet-30a ( + )-igf-ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins . 5
  sds-page分析表明，重组质粒pet-32a(+)-igf-在iptg诱导下表达分子量约30kda的融合蛋白，但其表达量不高，约为菌体总蛋白的10左右；重组质粒pet-30a(+)-igf-在iptg诱导下表达分子量约14kda的重组蛋白，融合蛋白表达量约占菌体蛋白总量的35。
• The recombinant expression plasmid was transformed into bl21 ( de3 ) and gene expression was induced by administration of iptg . expression was detected by sds-page and confirmed by western blot analysis, which shows that the yield of the target protein was approximately 30 % of the total bacterial protein
  将这个表达载体转化入大肠杆菌表达菌株bl21(de3)中，iptg诱导培养后，经sds-page和western杂交实验检测可知特异性表达带约在44kd的位置，其表达量大约占细菌总蛋白的30左右。